DeTOKI identifies and characterizes the dynamics of chromatin TAD-like domains in a single cell

DeTOKI identifies and characterizes the dynamics of chromatin TAD-like domains in a single cell

Blog Article

Abstract Topologically associating domains (TAD) are a key structure of the 3D Eye serum mammalian genomes.However, the prevalence and dynamics of TAD-like domains in single cells remain elusive.Here we develop a new algorithm, named deTOKI, to decode TAD-like domains with single-cell Hi-C data.

By non-negative matrix factorization, deTOKI seeks regions that insulate the genome into blocks with minimal chance of clustering.deTOKI outperforms competing tools and reliably identifies TAD-like domains in single cells.Finally, Novelty Products we find that TAD-like domains are not only prevalent, but also subject to tight regulation in single cells.